The objective of the national hydrogen and fuel cell R&D program is to help industry develop technologies to produce, store, transport and use hydrogen made from renewable resources in quantities large enough, and at costs low enough, to compete with traditional energy sources such as coal, oil and natural gas.  The bacterium Bacillus smithii, ATCC 55404, produces H₂ and CO₂ from CO and water through the well-known water-gas shift reaction.  B. smithii does not require light as an energy source for growth, and does not utilize CO as a growth substrate.  Thus, the bacterium can channel essentially all of the CO (with water) to H₂ production.   Batch serum bottle experiments were conducted under kinetically limited conditions to obtain cell (X), growth-limiting substrate (glucose, S₁), reaction substrate (CO, S₂) and product (H₂, P) concentrations with time.  The data were used to determine and model the specific growth rate (μ), the specific hydrogen production rate (ν), the specific carbon monoxide uptake rate (q), the yield of hydrogen from carbon monoxide (Y_P/S2) and the yield of cells from glucose (Y_X/S1).