Monday, May 5, 2008
6-29

Enzymatic Hydrolysis of Lignocellulosic Biomass for Bioethanol Production – A Study on Its Degradation Mechanisms, Kinetic models and Economical Impact

Boutros Fouad Sarrouh, Juan Daniel Rivaldi, and Silvio Silverio da Silva. Department of Biotechnology, Escola de Engenharia de Lorena, Universidade de São Paulo, Estrada Municipal do Campinho, s/n CEP 12.602-810, Lorena, Brazil

The potential importance of cellulose hydrolysis in the context of conversion of plant biomass to fuels and chemicals is widely recognized. Cellulose hydrolysis also represents one of the largest material flows in the global carbon cycle. The kinetics of cellulose hydrolysis is been widely studied, and Michaelis-Menten types of rate expressions with substrate or product inhibition terms have been proposed to describe the observed reaction kinetics. Also measured parameters for cellulase components and substrates could in principle be incorporated into models used to predict the behavior of multicomponent cellulase enzyme systems. Once a quantitative model is validated, it can be used to rapidly formulate new hypotheses of significance in both fundamental and applied contexts. The current work presents a detailed state of arte study on the degradation mechanisms of the enzymatic hydrolysis of lignocellulosic residues for bioethanol production, where different kinetic models for enzymatic hydrolysis are presented. Parameters such as enzyme adsorption, enzymatic inhibition and Beta-glucosidase adsorption are also studied. We suggest that more studies needed to be achieved concerning the functional modeling of enzymatic hydrolysis of cellulose. Models based on application of these and other methodologies to relate changes in substrate properties to rates of primary and secondary mediated by various cellulases and cellulose systems over the course of reaction appear to be a promising direction for future research. Acknowledgments: FAPESP and CNPq

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