Monday, May 5, 2008
7-19

Proteome characteristics of succinic acid overproducing bacterium, Mannheimia succiniciproducens

Jeong Wook Lee1, Hyohak Song1, Sol Choi1, Jong Shin Yoo2, and Sang Yup Lee1. (1) Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Department of BioSystems and Bioinformatics Research Center, Institute for the Biocentury, Korea Advanced Institute of Science and Technology (KAIST), 335 Gwahangno, Yuseong-gu, Daejeon, South Korea, (2) Korea Basic Science Institute, 52 Yeoeun-dong, Yuseong-gu, Daejeon, South Korea

A capnophilic rumen bacterium, Mannheimia succiniciproducens MBEL55E, isolated from bovine rumen is an efficient succinic acid producer. Recently, a genetically engineered succinic acid overproducing mutant, M. succiniciproducens LPK7, was developed based on full genome sequence. 2-DE and LC-MS/MS were used to analyze proteome of the mutant cells at the exponential and stationary phases. The results were compared with those of the wild type strain (MBEL55E) to elucidate the global physiological and metabolic changes responsible for succinic acid overproduction. Comparative proteomic analyses between the MBEL55E and the mutant strain showed the apparent differences in 87 and 69 protein spots at the exponential and stationary phases, respectively. As the mutant cells grow, the expression levels of 58 proteins also changed. The results could allow us to understand the global protein changes related with succinic acid overproduction. Moreover, several features, indispensable for further improving the succinic acid producers by rational metabolic engineering, will be described in detail. [This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (2005-01294). Further supports by the LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.].

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