Monday, May 5, 2008
7-16

Effects of dissolved CO2 levels on the growth of Mannheimia succiniciproducens and succinic acid production

Hyohak Song, Jeong Wook Lee, Sol Choi, and Sang Yup Lee. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Department of BioSystems and Bioinformatics Research Center, Institute for the Biocentury, Korea Advanced Institute of Science and Technology (KAIST), 335 Gwahangno, Yuseong-gu, Daejeon, South Korea

This study presents the metabolic responses of Mannheimia succiniciproducens to the different dissolved CO2 concentrations (0 to 260 mM). The cell growth and succinic acid production rates increased proportionally as the dissolved CO2 concentration increased from 8.74 to 141 mM. Also, the yields of biomass and succinic acid on glucose obtained at the dissolved CO2 concentration of 141 mM were 1.49 and 1.52 times higher, respectively, than those obtained at the dissolved CO2 concentration of 8.74 mM. The additional CO2 sources provided in the form of NaHCO3, MgCO3, or CaCO3 had positive effects on cell growth and succinic acid production. While, cell growth inhibition was observed when excessive bicarbonate salts were added. We also found that PEP carboxylation by PEP carboxykinase (PckA) is the most important for succinic acid production as well as the growth of M. succiniciproducens since it provides the additional ATP. [This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (2005-01294). Further supports by the LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.].

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