Monday, May 5, 2008
7-13

Screening of microorganisms for bioconversion of limonene to a-terpineol

Ieda Rottava1, Camila Grando2, Geciane Toniazzo2, Altemir J. Mossi2, Ingrid C. R. Costa1, Rogério L. Cansian2, and Octavio Augusto Ceva Antunes3. (1) Bioquímica, Universidade Federal do Rio de Janeiro, Instituto de Química, CT, Bloco A, Lab. 641, Rio de Janeiro, 21.945-970, Brazil, (2) Ciências Agrárias/Eng de Alimentos, URI - Campus de Erechim, Av. Sete de Setembro, 1621, Erechim, 99700-000, Brazil, (3) Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Instituto de Química, CT, Bloco A, Lab. 641, Rio de Janeiro, 21945-970, Brazil

The limonene, a cheap and readily available natural substrate, which can be used for the production of more valuable natural flavour compounds of interest for the industries of cosmetics, druggist and foods. The a-terpineol is the most important of the monocyclic monoterpene alcohols. The objective of this work was to carry through screening of microorganisms, from rejects of the citric juice industry, and other sources of limonene, for bioconversion of this. The election was carried through with the isolation of diverse microorganisms in plates with Potato Dextrose Agar. To verify the enzyme presence that degrades limonene in the isolated microorganisms, it was used minimum media more limonene, in electromagnetic agitation. Fermentation in orbital agitator was become fulfilled, using minimum media and limonene (1.5%, v/v), with the microorganisms that had presented resulted positive in the previous test. The products gotten in the bioconversion process (a-terpineol), had been characterized by GC/MS with yield of 30 %.

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