Monday, May 5, 2008
7-10
Changes in proliferation characteristics and a filter paper degrading ability of the Trichoderma strain, B5, after the second colchicine treatment
Hideo Toyama, Hiroshi Kawamata, and Satoshi Minamisawa. Food Science for Health, Minamikyushu University, Kirishima 5-1-2, Miyazaki, 880-0032, Japan
In this study, we attempted to observe the changes in proliferation characteristics and a filter paper degrading ability of the Trichoderma strain B5 after the second colchicine treatment. The strain B5 is selected from the colchicine-treated conidia of T. reesei Rut C-30 using the double layer selection medium and this strain possesses superior proliferation characteristics and degrading ability of a filter paper comparing with the original strain.
The green mature conidia of the strain B5 were swollen and treated with colchicine solution. These treated conidia were incubated in the double layer selection medium followed by isolation of several strains.
Every selected strain appeared on the surface of the selection medium earlier than those derived from T. reesei Rut C-30. These selected strains showed superior proliferation characteristics on the Avicel plates comparing with the strain B5 and the original strain.
Moreover, these selected strains showed superior degrading ability of a filter paper comparing with the strain B5 and the original strain.
From these results, we concluded that additional colchicine treatment can cause increase in proliferation rate on the Avicel plates and the degrading ability of a filter paper.
The green mature conidia of the strain B5 were swollen and treated with colchicine solution. These treated conidia were incubated in the double layer selection medium followed by isolation of several strains.
Every selected strain appeared on the surface of the selection medium earlier than those derived from T. reesei Rut C-30. These selected strains showed superior proliferation characteristics on the Avicel plates comparing with the strain B5 and the original strain.
Moreover, these selected strains showed superior degrading ability of a filter paper comparing with the strain B5 and the original strain.
From these results, we concluded that additional colchicine treatment can cause increase in proliferation rate on the Avicel plates and the degrading ability of a filter paper.
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See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)