Monday, May 5, 2008
6-20
The effect of corn stover pretreatment on enzyme inhibition
Don L. Higgins, Erin Quatrini, Feng Xu, and Mads Torry-Smith. BioFuels R&D, Novozymes North America, 77 Perry Chapel Church Rd, Franklinton, NC 27525
The generation of soluble inhibitory compounds during biomass pretreatment has been well documented, and the extensive list of compounds includes aliphatic acids, furans, phenolic compounds and heavy metals. The characteristics of the pretreatment process directly affect the presence and concentration of inhibitors in the whole, pretreated biomass slurry. It is well known that certain inhibitory compounds affect the speed and productivity of fermentation, but, in addition, a subset of these compounds adversely affect cellulase activity. Cellulase inhibition, whether by specific or non-specific mechanisms, will increase the amount of enzyme required for complete cellulose hydrolysis, thereby driving the cost of the process upwards. This demonstrates the importance of integrating the pretreatment, enzyme hydrolysis and fermentation operations early during process development. Less research has been performed on the role of specific pretreatment processes in the generation of potent enzyme inhibitory compounds. This presentation will focus on the characterization of enzyme inhibition from corn stover pretreated by different pretreatment methods and will reveal the most potent cellulose-inhibiting substrates.
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See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)
See more of General Submissions
See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)