Sunday, May 4, 2008
3-52

Effect of wet disk milling-pretreatment without sulfuric acid for enzymatic hydrolysis of rice straw

Akihiro Hideno, Hiroyuki Inoue, Kenichiro Tsukahara, Seiichi Inoue, Takashi Endo, and Shigeki Sawayama. Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology, 2-2-2 Hiro-suehiro, Kure, Japan

Recently, rice straw which can be a potential source for ethanol production has been attracted a lot of interest in Japan. We investigated a wet disk milling-pretreatment without sulfuric acid of rice straw, and compared with the conventional methods such as a ball milling, and a hydrothermal treatment in this study. The rice straw cut out to less than 2 mm by the cutter mill was pretreated by the wet disk milling (10 cycles in continuous treatment), the ball milling (60 min) and the hydrothermal treatment (160 °C or 180 °C, 30 min), respectively. The pretreated materials were hydrolyzed by the mixture of the Acremonium cellulase (4-40 FPU/g dry rice straw) and the commercial hemicellulase Optimash BG. The glucose yields obtained from the wet disk milling, the ball milling and the hydrothermal treatment were 290-350 mg/g dry sample, 300-370 mg/g and 270-320 mg/g, respectively. The xylose yield obtained from these pretreatment were 90 mg /g, 90-120 mg/g and 50-110 mg/g, respectively. These results indicate that the wet disk milling was effective for enzymatic hydrolysis as well as other conventional pretreatments. Moreover, the wet disk milling is not able to treatment continuously but also can save energy as compared with ball milling. The wet disk milling can be an economical pretreatment for enzymatic hydrolysis of rice straw. This work was supported by the Resional Biomass Energy Project, Ministry of Agriculture, Forestry and Fisheries, Japan.

See more of Poster Session One
See more of General Submissions

See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)