Sunday, May 4, 2008
2-56

Metabolic profiling of white-rot fungi, Phanerochaete chrisosporium andDichomitus squalens cultured with lignocellulose biomass

Jin Seop Bak, Division of Food Bioscience and Technology, Korea University, College of Life Sciences and Biotechnology, Anam-dong, Seongbuk-gu, Seoul, South Korea and Kyoung Heon Kim, School of Life Science & Biotechnology, Korea University, College of Life Sciences and Biotechnology, Anam-dong, Seongbuk-gu, Seoul, South Korea.

White rot fungi, Phanerochaete chrisosporium and Dichomitus squalens have long been recognized to possess the lignin-degrading activity.  In this study, to exploit the capability of the white rot fungi’s lignin degradation for tackling the recalcitrance of the lignocellulosic biomass, the physiological characteristics of the fungi on cellulose or lignocellulose biomass were investigated by the metabolic profiling.  The cultivation of the white rot fungi was carried out on synthetic media containing a variety of carbon, nitrogen, trace minerals, and vitamin sources.  Both the exometabolome and endometabolome were analyzed by using a quadruple type of GC/MS after appropriate quenching and extraction procedures.  The mass spectrometric data were statically analyzed by principal component analysis (PCA) and partial least squares discriminant analysis (PLSDA) to find how the environment conditions (carbon, nitrogen and oxygen supply and so on) affect the profiles of endometabolome and exometabolome.  This microbial metabolomics approach is expected to provide the holistic view of the response of the fungal metabolism to the culture environment, especially the presence of lignocellulosic biomass in media.


Web Page: www.bioethanol.or.kr, www.metabolomics.or.kr

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