Monday, May 5, 2008
12-19

Hydrolysis of Steam Explosion Pretreated Sugarcane Bagasse Using Cellulase and Cellobiase

Liliane Pires Andrade1, Giovana F. Dionisio1, Joao Moreira Neto1, Cleide M. F. Soares2, Flavio F. Moraes1, and Gisella M. Zanin1. (1) Chemical Engineering Department, State University of Maringa, Av. Colombo, 5790, BL E-46, Maringa - PR, 87020-900, Brazil, (2) Instituto de Tecnologia e Pesquisa, Universidade Tiradentes, Av. Murilo Dantas, 300, Farolandia, Aracaju - SE, 49032-490, Brazil

Currently, Brazil is faced with the prospect of a significant increase in the demand for fuel ethanol. This is a result of three main factors: (i) increasing domestic consumption of hydrated alcohol, (ii) expansion of Brazilian alcohol exports on the basis of a growing worldwide interest in the mixture of gasoline and alcohol, and (iii) the Brazilian option for the production of biodiesel using ethanol for the transesterification of vegetable oils. To meet this increasing demand, without reducing the area dedicated to food production, the alternatives are to increase the alcohol productivity per hectare of sugarcane plantation and to develop an economically viable technology to use the sugarcane bagasse surplus for the production of additional ethanol. The sugarcane bagasse is an abundant, renewable resource in Brazil, and it contains a high level of cellulose, which can be employed for the production of ethanol. This work objective was to evaluate the influence of the ratios of sugarcane bagasse:cellulase enzyme and bagasse:cellobiase enzyme on the bagasse hydrolysis reaction, which produces fermentescible sugars as glucose. The tests showed that the maximum production of glucose was reached when a 100 mL volume of a 3% (w/v) sugarcane bagasse was hydrolized with 1.25 mL cellulase (20.84 U/100 mL) and 0.625 mL cellobiase (0.98 U/ 100 mL).

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