The world’s biodiesel marked is in constant increasing. As resulted of its high volume production, large amount of glycerol, major byproduct in the biodiesel industry, is being generating. Normally, glycerol valorization includes direct chemical transformation to useful compounds as feedstock in food, cosmetic, pharmaceutical and chemical industries. However, the purification treatments are too expensive for small and medium size biodiesel producers, due to this fact; more and more quantity of glycerol could be accumulated or discharged in the environment without any treatment. The microbial conversion of glycerol to different products, such as ribonucleotides, single cell protein, citric acid or ethanol, could constitute an alternative to turn biodiesel production economically feasible. In this work, crude glycerol (25 g/L) was used as substrate for the growth of the yeast Hansenula anomala (pH 5.5; 200 rpm; 30 ^oC). It was found a relatively high cell dry weight concentration (5.0 g/L) after 24 hours of fermentation. Furthermore, it was assayed a considerably amount of total RNA excreted in the medium (46.4 mg/L) and 3.4 mg/g of RNA after 24 h autolysis of the cell (pH 7,4 ; 60 ^oC). Besides, ethanol (3.6 g/L), acetate (0.3g/L), citrate (0.6 g/L) and succinate (1.4 g/L) were formed after 72 h. The above results indicate that crude glycerol could be a promising alternative for the production of added-value products.Keywords: glycerol, microorganism, fermentation, bioproduct.

Acknowledgments: CAPES, CNPq: CAPES, CNPq: CAPES, CNPq