In this work two complementary chromatographic methods are developed to analyze sugar profiles (monosaccharides and disaccharides) and some organic acid that are present in different biomass feedstocks and wastes.

 Both methods are based in cation exchange chromatography, first one with hydrogen (H⁺) and second one with lead (Pb²⁺) ionic forms, and are operated with a RI detector under isocratic elution conditions.

 One of the major drawbacks of the first method is that for certain sugars, a low resolution can be obtained. In fact, mannose, galactose, xylose and fructose elute together in one peak. However, other different sugars can be quantified with a good resolution (cellobiose, glucose, arabinose, ribose) and some organic acid like citric, malic, galacturonic, glucuronic and oxalic.

 Nevertheless, the second method allows a good enough quantification of the sugars that can not been well resolved in the first ones: sucrose, glucose, xylose, galactose, arabinose, mannose and fructose.

 Finally, we have confirmed the validity of both methods comparing the RSD (Relative Standard Deviation) values of sugars that can be properly quantified with both methods.

 In short, with these two complementary methods we have obtained a complete and precise profile of the sugars and certain organic acids that are present in some feedstocks and wastes.