Monday, May 5, 2008
12-13

Cellulase and hemicellulase production of the hyper-producing mutant of Acremonium cellulolyticus

Xu Fang, Shinichi Yano, Hiroyuki Inoue, and Shigeki Sawayama. Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology, 2-2-2 Hiro-suehiro, Kure, Japan

Cellulase and hemicellulase are the enzymes that can hydrolyze lignocellulosic biomass to fermentable sugars. Acremonium cellulolyticus secretes sufficient amounts of cellulolytic enzymes to completely convert cellulose materials to glucose. Cellulase from A. cellulolyticus has notably higher β-glucosidase activity than those from Trichoderma species.

  To efficiently produce cellulase and hemicellulase, A. cellulolyticus CF-2612 mutant strain was obtained with random mutagenesis. The enzyme activities in the supernatant of the culture as crude enzyme preparations were measured, followed by shake flask culture at 30oC using Solka Floc as the carbon source. The FPase, avicelase, and β-glucosidase activities of crude enzyme from CF-2612 strain were significantly higher than those of the parent strain. FPase activity (U/ml) and FPase-specific activity (U/mg protein) reached 18.0 and 1.0, respectively, using the batch culture with 5% Solka Floc for 5 days in a 2-L jar fermenter. When the crude enzyme from CF-2612 strain was used for saccharification of pretreated rice straw, glucose, xylose, arabinose and mannose were released. This result suggests that not only cellulase but also hemicellulase are secreted by CF-2612 strain.

  In conclusion, A. cellulolyticus CF-2612 strain has an ability to efficiently produce cellulase and hemicellulase for hydrolysis of biomass.

  [This study was supported by a Greenhouse gas mitigation technology development program of the Ministry of Environment of Japan.]


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