Monday, May 5, 2008
6-17
Cloning of Cellulase and Regulation Factor Genes in Penicillium decumbens and Analysis of the Mutation Mechanism of Strain JU-A10
Xianyun Sun, Ziyong Liu, Xiaomin Wei, Xin Song, and Yinbo Qu Sr.. State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan road, Jinan, 250100, China
Cellulolytic fungus, Penicillium decumbens JU-A10 was a catabolite-repression-resistant mutant, which was obtained by physical and chemical mutagenesis of strain 114-2. Recently, strain JU-A10 has been successfully applied to cellulase preparations and cellulosic ethanol production at an industrial scale in China
Five cellulase genes (cbh1, cbh2, egl1, egl2 and bgl1) and two regulation factor genes (creA and ace1, encoding repressor of cellulase and xylanase expression) in P. decumbens were cloned by TAIL-PCR and degenerate PCR. Previous study shown that the catabolite repression-resistant character of strain JU-A10 may be caused by the change of energy-yielding metabolism or the mutation of catabolite repressors. By comparing the sequences and transcription of the seven genes in P. decumbens 114-2 with its mutant JU-A10, we found that the catabolite repression-resistant character of strain JU-A10 were caused by neither the mutation of these cellulase genes or upstream regulatory sequence, nor the mutation of catabolite repressors CRE A or ACE Ι. Based on comprehensive analysis, it is proposed that the catabolite repression-resistant character of strain JU-A10 is caused by the change of energy-yielding metabolism. Further verification is under way.
Five cellulase genes (cbh1, cbh2, egl1, egl2 and bgl1) and two regulation factor genes (creA and ace1, encoding repressor of cellulase and xylanase expression) in P. decumbens were cloned by TAIL-PCR and degenerate PCR. Previous study shown that the catabolite repression-resistant character of strain JU-A10 may be caused by the change of energy-yielding metabolism or the mutation of catabolite repressors. By comparing the sequences and transcription of the seven genes in P. decumbens 114-2 with its mutant JU-A10, we found that the catabolite repression-resistant character of strain JU-A10 were caused by neither the mutation of these cellulase genes or upstream regulatory sequence, nor the mutation of catabolite repressors CRE A or ACE Ι. Based on comprehensive analysis, it is proposed that the catabolite repression-resistant character of strain JU-A10 is caused by the change of energy-yielding metabolism. Further verification is under way.
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See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)