Sunday, May 4, 2008 - 2:30 PM
2-02

The Development of TM242: A Novel Thermophilic Bacillus Capable of High Yield Ethanol Production

K. Eley1, R. Cripps1, B. Rudd2, M. Todd2, and T. Atkinson1. (1) TMO Renewables Ltd, 40, Alan Turing Road, Guildford, United Kingdom, (2) Novacta Biosystems Ltd, Broadwater Road, Welwyn Garden City, United Kingdom

The efficient conversion of lignocellulosic biomass to liquid transport fuels such as ethanol has become a major industrial goal in the effort to reduce dependency on fossil fuels and reduce CO2 emissions. Fermentation of biomass hydrolysates has been hampered by the lack of suitable microorganisms that can effectively metabolise all of the hexose and pentose sugars present in these hydrolysates. Strategies to address this have included engineering yeast to metabolise pentose sugars, and the modification of a number of bacterial species to increase ethanol yields. Thermophilic microorganisms have a number of potential advantages for the development of fermentation processes for alcohol as a biofuel. High growth temperature favours high rates of growth and ethanol productivity and also reduces the risk of contamination which continues be a significant problem in large-scale commercial operations.


The parent strain of TM242, a Geobacillus sp., was chosen because of its ability to metabolise a wide range of biomass derived monomeric and oligomeric sugars. It is a facultative anaerobe capable of growth between 40-70oC and can ferment both hexose and pentose sugars to generate lactate, formate, acetate and relatively low amounts of ethanol as products. Newly developed metabolic engineering tools have been applied to this organism to alter the expression of key enzymes to produce TM242 in which acid production is essentially eliminated and ethanol is efficiently produced at yields approaching the theoretical maximum value. This presentation will outline the metabolic steps involved in the creation of TM242 and illustrate the performance benefits at each step.



Web Page: www.tmo-group.com