The ability of the corn dry milling coproduct thin stillage derived from ethanol fermentation to support production of the polysaccharide curdlan was examined. A number of commercial uses exist for curdlan such as food and beverage applications. The bacterium Agrobacterium sp. ATCC 31749 synthesizes the polysaccharide in a nitrogen-limiting medium containing excess carbon source. In this study, the bacterium was grown in a phosphate-buffered minimal medium (pH 6.8) that contained thin stillage (from two different sources) as a source of carbon and nitrogen. With carbon limiting in the thin stillage, the effect of supplementing 3% corn syrup to the medium was also explored. The strain was grown in shake flask cultures for up to 120 hours at 30^oC. To inoculate each culture, a culture containing the same medium grown for 48 hours was used.  To determine curdlan and biomass production, gravimetric determinations were utilized. The strain could produce the polysaccharide from the medium containing the thin stillage alone. The highest curdlan concentration was observed after 96-120 hours 30^oC independent of the source of thin stillage used. With curdlan production being further elevated after 120 hours at 30^oC when the medium was supplemented corn syrup, carbon appeared to be limiting in either source of thin stillage. Biomass production by the strain grown on the corn syrup-containing medium generally was similar to its production on the unsupplemented medium. In summary, a medium containing the thin stillage supported curdlan production although bacterial polysaccharide production could be further elevated by corn syrup supplementation.