Monday, May 5, 2008
6-43
Enzymatic conversion of sucrose to invert sugar using a membrane reactor
Michele Vitolo and Ester Junko Tomotani. Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, B.16, 05508-900, São Paulo, Brazil
The aim of this work was to evaluate the performance of soluble and insoluble invertase for the conversion of sucrose to invert sugar through a continuous process using the membrane reactor (MR) coupled with a 100kDa-cutoff ultrafiltration membrane (UF-MR) or a 5μm-pore-size microfiltration membrane (MF-MR). Invertase was immobilized by adsorption on anionic polystyrene-divynilbenzene beads. The 10mL-membrane reactor (UF-MR or MF-MR) was fed continuously with a 2.5mM sucrose solution at feeding rate of 1.6 h-1, being the temperature and agitation maintained at 30oC and 100 rpm, respectively. At least, a continuous 20h-steady-state regime was attained in all tests. The yields (expressed as percent of sucrose converted) and reaction rates (expressed as mmol/h.menzyme) for sucrose conversion to invert sugar were 100%, 84% and 99% and 0.23, 0.88 and 1.02, respectively, for soluble-UF-MR, insoluble-UF-MR and insoluble-MF-MR. No leakage of enzyme from the support was detected. Insoluble-MF-MR had similar yield and reaction rate 77% higher than that of soluble-UF-MR. The improved performance of insoluble-MF-MR over soluble-UF-MR would probably be due to differences of structural and/or chemical nature of the membranes, leading to different internal flux patterns, which, at the end, would affect the reaction rate and the yield. The data showed that the membrane reactor was suitable for sucrose hydrolysis.
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See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)
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See more of The 30th Symposium on Biotechnology for Fuels and Chemicals (May 4 -- 7, 2008)