Invited Oral Abstract Presentation
Making scents of waste: Fermentative production of high-value flavour and aroma compounds from food industry by-products
Ms. Melodie Lindsay, Prof. David Greenwood, Mrs. Ninna Granucci and Silas Villas-Boas, The University of Auckland, Auckland, New Zealand
2017 SIMB Annual Meeting and Exhibition
Perfume and flavouring agents have been valued by civilisations for thousands of years. Today, modern fragrance and flavour ingredients can comprise up to 5% of the total cost of many consumer goods/products. This USD 25 billion industry continues to expand in particular, due to consumer demand for natural alternatives to artificial additives. We know that fermentation can be used to naturally enrich the volatilome of many organic substrates for example, grape juice vs wine. Therefore, microbial bioconversion of readily available primary industry waste may be an attractive solution to naturally produce fragrance and flavour chemicals compared with expensive traditional methods. We have completed over 50 different fermentations using substrate derived from primary industry waste including: juice and wine by-products, olive pomace and waste onions. A range of fungi were used to conduct laboratory-scale liquid-, solid- and/or submerged-state fermentations. For each, sterile substrate was inoculated with a single fungal strain. The volatilome of both fermented and unfermented substrate was then analysed using headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-mass spectrometry (GC-MS). Fermented profiles were compared to unfermented substrates to identify de novo production of flavour and aroma compounds. Volatile profiling showed that hundreds of compounds were produced, of which many were identified using the NIST2014 library as well as an in-house library. Several valuable compounds were produced including those with mint, rose, cherry and honey characters. These are now the focus of optimisation and scale-up trials.
Invited Oral Abstract Presentation
Pushing productivities for lactate fermentation on lignocellulosic hydrolysates in membrane bioreactor mode
Wouter Van Hecke1, Suzanne Verhoef2, Wim Groot2, Marija Saric3, André de Haan4 and Heleen De Wever1, (1)VITO, Mol, Belgium, (2)Corbion, Gorinchem, Netherlands, (3)ECN, Petten, Netherlands, (4)TU Delft, Delft, Netherlands
2017 SIMB Annual Meeting and Exhibition
Polylactic acid (PLA) became the first bio-based polyester manufactured on an industrial scale. Its production price is mainly affected by feedstock costs and by the efficiency of the production process. Lignocellullosic biomass offers potential for cost reductions on the longer term. For high volume, low margin products, continuous fermentation technology offers advantages in comparison to (fed-)batch technology. The aim of our work was to investigate continuous lactate fermentations at increased cell densities on simulated lignocellulosic hydrolyzate and wheat straw hydrolyzates using Ca(OH)2 suspensions as base.
To this end, a set-up with external tubular membranes was designed and constructed and long-term continuous and axenic fermentations were run in membrane bioreactor mode.
Fouling of the membranes proved to be a major bottleneck in the process. Automatization circumvented this problem, allowing constant operating conditions throughout extended periods of time.
Maximal productivities of 30.5 g.kg-1.h-1 could be reached in the prototype combined with an average lactate concentration of 85.4 g.kg-1 and complete consumption of C5 and C6 carbohydrates.
Hence, significant performance improvements in comparison to batch and (regular) continuous conditions were demonstrated.
Invited Oral Abstract Presentation
Advanced alcohol recovery methods
Dr. Scott Kohl, White Energy, Plano, TX, USA
2017 SIMB Annual Meeting and Exhibition
The fermentative production of alcohol has made significant advancements over the past decade. These improvements have been able to raise the terminal concentration of alcohol in the end product as well as produce non-ethanol alcohols at sufficient yields and speed to be industrially relevant. One of the newer advents in commercial alcohol production is novel recovery techniques. A vacuum recovery method has been developed for both ethanol and butanol production. This process has advantages in that the alcohol product is removed from the fermentation broth during ethanol production. This process substantially removes end product inhibition allowing the fermentation process to proceed faster and to higher “net†titre that previously available. Discussion of this technology at both laboratory and full-scale implementation will be given along with comparison to the standard finished beer distillation process used in most commercial applications.
Invited Oral Abstract Presentation
Break
2017 SIMB Annual Meeting and Exhibition
Invited Oral Abstract Presentation
Computational bioseparation process development
Marcel Ottens, Delft University of Technology, Delft, Netherlands
2017 SIMB Annual Meeting and Exhibition
With the increase in computational power over the last decades, the use of modeling and simulation in process design for (petro)chemical industry has become common ground. Computational tools like ASPEN are standard in the design and operational analysis of (petro)chemical plants. However, in the bio pharmaceutical field, such modeling and simulation techniques are only recently being investigated for use and (potential) implementation. Being the workhorse of purification in the biopharmaceutical industry, chromatography is a good candidate for this modeling approach. Detailed mechanistic models describing chromatographic separation behavior are available, and software to simulate chromatography is becoming more and more available (i.e. DelftChrom, CADET, etc.). A bioseparation process normally consists of multiple chromatographic and conditioning steps, hence, an extreme large design space needs to be investigated. This may lead to prohibitive simulation times, even on state-of-the-art fast computers, when only mechanistic models are used. This presentation will show the implementation of a hybrid bioseparation process design approach using a combination of mechanistic models, artificial neural networks and high throughput experimentation for process development and optimization of the production of industrial relevant biologicals.
Invited Oral Abstract Presentation
CANCELLED; Advances in downstream processing of biotherapeutic proteins from microbial sources
Seth Fisher, Cytovance Biologics, Oklahoma City, OK, USA
2017 SIMB Annual Meeting and Exhibition
Downstream Process of Biotherapeutic proteins involves multiple unit operations including cell homogenization and clarification, typically several chromatography steps, and Tangential Flow Filtration for final formulation. In addition, some microbial products need to be refolded. This presentation will highlight advances in each area as well as challenges in incorporating new technology.