The agriculture has been characterized by the intensive use of chemical fertilizers to maintain high yields, without taking into account its negative effects. This generates the need to develop technologies which favor the nutrition of plants. Due of this the objective of this work was isolate native rhizobacteria from soils from central Mexico and evaluate their plant growth promoting ability in potato cultivation (Solanum tuberosum). 120 bacteria were isolated from maize (56) and potato (64) rhizosphere, and purified, characterized and preserved at -80 ° C. Their effect on germination and root elongation in tomato seeds was evaluated. A group of 4 bacteria was selected according to their capacity to produce AIA, ACC desaminase and/or solubilize phosphates, tolerance to agrochemicals and growth promoting effect. The isolated selected was identified according to the sequence analysis of the 16S rRNA gene. The results showed that the treatments inoculated with the strains PT41PS and MT53PS showed a significant difference in the Germination Index and significantly increasing in height (67%), dry weight of stem, root and tuber (three more times) of the plants with respect to the commercial product and to the uninoculated control. The strains identified as Enterobacter cloacae and E. asburiae produces AIA (7.97 and 6.53 mg L^-1), ACC deaminase (0.137 and 0.015 mM α-ketobutyrate) and solubilizes phosphate (SI=4.95 and 2.38 respectively). Both strains tolerate high concentrations to different agrochemicals. This suggests that they can be used as a biofertilizer for potato cultivation under greenhouse conditions in the central region of Mexico.