Industrial cell-free bioprocessing

GreenLight Biosciences has developed a flexible bio-manufacturing platform that leverages proprietary breakthroughs in Cell-free Bioprocessing. Cell-free Bioprocessing takes full advantage of the high selectivity, yield, and sustainability of biological catalysts without the burden of maintaining a living organism under more rigid physiological conditions. Bioprocess development is accelerated through the rapid testing of precise biocatalytic system variation without the need to reengineer organisms or manage negative impacts on cell viability. GreenLight’s approach eliminates the need to keep a host organism alive while preserving the biological manufacturing capabilities of the cell.

This talk with focus on how Cell-free Bioprocessing addresses some of the most challenging aspects of current bioproduction efforts. These include effectively re-routing carbon without negatively affecting cell growth, managing reducing equivalents from over-active pathways, and avoiding cytotoxic effects of desired products or high product titers. Emphasis will be placed on technology that enables the highly selective elimination, or modulation, of enzymatic activities derived from the engineered organism. The ability to precisely ‘tune’ cell-free biocatalytic activities allows for effective control of carbon flux from feedstock to product for achievement of maximal yield and productivity.