S36 Production of long chain terminal alcohols through the ‘+1’ pathway: combining enzyme engineering, atrain development and fermentation optimization to accelerate development
Monday, July 25, 2016: 2:00 PM
Waterbury, 2nd Fl (Sheraton New Orleans)
C. Stowers*, M. Devarapalli and N. Mouncey, Dow AgroSciences LLC, Indianapolis, IN; D. Rosenfeld, A. Arthur and B. Kolthammer, Dow Chemical Company, Freeport, TX
C4 – C9 alcohols have a wide range of uses including fuels, lubricant additives, plasticizers and are also precursors to other important chemicals. Production of these alcohols through petrochemical means can be challenging since existing technologies such as linear alpha oligomerization are not selective. Production of non-Ziegler alcohols bring additional challenges since odd-chain petrochemical feedstocks are rare. These challenges present a unique opportunity for the development of a bioprocess to selectively produce C4-C9 alcohols. The Dow Chemical Company has developed novel enzymes and microorganisms for selective production of these alcohols through an integrated program using enzyme engineering, metabolic engineering and fermentation optimization. This presentation will summarize the development of this technology with a specific emphasis on strain construction and fermentation optimization.