P139 Accumulation of D-Glucose from pentoses by Escherichia coli
Sunday, August 2, 2015
Tian Xia1, Qi Han1, William V. Costanzo1, Yixuan Zhu1, Jeffrey L. Urbauer2 and Mark A. Eiteman1, (1)BioChemical Engineering, University of Georgia, Athens, GA, (2)Department of Chemistry, University of Georgia, Athens, GA
Escherichia coli unable to metabolize D-glucose (knockouts in ptsG, manZ, glk) accumulates a small amount of D-glucose (yield of about 0.01 g/g) during growth on the pentoses D-xylose or L-arabinose as a sole carbon source. Additional knockouts in zwf and pfkA genes encoding respectively D-glucose-6-phosphate 1-dehydrogenase and 6-phosphofructokinase I (E. coli MEC143) increased accumulation to greater than 1 g/L D-glucose and about 100 mg/L D-mannose from 5 g/L D-xylose or L-arabinose.  Knockouts of other genes associated with interconversions of D-glucose-phosphates demonstrate that D-glucose is formed primarily by the dephosphorylation of D-glucose-6P.  Under controlled batch conditions with 20 g/L D-xylose, MEC143 generated 4.4 g/L D-glucose and 0.6 g/L D-mannose.  The results establish a direct link between pentoses and hexoses, and provide a novel strategy to increase carbon backbone length from five to six carbons by directing flux through the pentose phosphate pathway.

 Key words:     L-arabinose; D-glucose-6-phosphate 1-dehydrogenase; pentose phosphate pathway; 6-phosphofructokinase I; glucose-6P isomerase; D-xylose