P89 Producing pyrroloquinoline quinone in Escherichia coli through overexpression pqqA genes of Hyphomicrobium denitrificans
Sunday, August 2, 2015
Mr. Chongrong Ke1, Mr. Xianzhang Jiang1, Mrs. Lufang Zhong1, Prof. Yong Tao2 and Prof. Jianzhong Huang1, (1)College of Life Sciences, Fujian Normal University, Fuzhou, Fujian, China, (2)Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
Pyrroloquinoline quinone (PQQ) is a novel biofactor for several bacterial dehydrogenases, participating in numerous biological processes. The biosynthesis of PQQ is a complex process and is synthesized from a conserved tyrosine and glutamate located near the C-terminus of precursor peptide encoded by pqqA. H. denitrificans can naturally synthesize PQQ, equipped with five copies of pqqA genes. We expressed the pqqBCDE gene cluster with different pqqA from H.denitrificans in Escherichia coli, resulting in PQQ accumulation up to 13.45mg/L in the medium. Multiple pqqA genes were play significant roles and contribute differently in PQQ synthesis. Overexpression of pqqA gene showed positive effect on PQQ production.
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