Endophytic microorganisms, isolated from medicinal plants, are a promising source for new, bioactive compounds. Vochysia divergens is a medical plant common in South America, and widely used for its anti-bacterial activity. Strain LGMB259 was isolated from leaf tissue of V. divergens and classified as Miscrobispora sp. by 16S rRNA phylogenetic analysis. Strain LGMB259 was cultivated in R5A-medium, and one of its secondary metabolites showed high antibacterial activity (against Micrococcus luteus and Kocuria rosea), and moderate antifungal activity (against Colletotrichum gloesporioides, Saccharomyces cerevisiae). The compound was purified by Sephadex LH-20 and HPL chromatography. Based on the NMR (Figure 1) and HR-ESI-MS (m/z 239.0814 [M + H]+), the structure of this compound was confirmed as 1-vinyl-β-carboline-3-carboxylate (C14H10N2O2).
Figure 1: Selected 1H,1H-COSY (▬) and HMBC (→) correlations of 1-vinyl-β-carboline-3-carboxylate