BioBLU Single-Use Vessels and Reusable Glass Bioreactors

In recent years single-use bioreactors gained more and more importance for animal and human cell culture in all biopharmaceutical industries. Minimized setup times, eliminated cleaning procedures and therefore reduced labor time can sustainably accelerate bioprocess development. With the new BioBLU f line, specifically designed to meet the needs of fermentation, single-use bioreactors make their way to microbial applications in biopharma, food and cosmetics industries. Microbial applications make specific demands on the bioreactor design and functionality. Fermentation processes need much higher k_La values for proper mass transfer and suitable heating and cooling options as well.

This comparative study investigates the functionality and reliability of a BioBLU 0.3f single-use mini bioreactor and an autoclavable DASbox Mini Bioreactor (figure 2) in a small scale E.coli fermentation. Reproducible process control was achieved with parallel operated BioBLU 0.3f single-use and reusable glass vessels, both used in an Eppendorf DASbox Mini Bioreactor System. Fermentation of E. coli K12 led to very comparable results thus proving the tested single-use vessels to be an appropriate tool to accelerate microbial process development and shorten time-to-market in all industries related to microbial production processes.