Lysozyme is a lytic enzyme and it has anti-bactericidal effect. Although lysozyme was found in many sources including plants and animals, human lysozyme is safer for food industry due to immunological issues. Up to now, human lysozyme production by genetically modified Kluyveromyces lactis K7 has been studied in suspended cell and biofilm reactor with plastic composite supports. Although biofilm reactor provides higher productivity, the production rate decreased after a certain time during fermentation due to product inhibition or degradation. Therefore, the goal of this study is to enhance human lysozyme production by including an online recovery system in biofilm reactor, which will eliminate the product inhibition or degradation. The effective recovery process can be achieved by the optimization of adsorption and desorption conditions. This study included two phases; evaluation of the adsorption and desorption conditions for the human lysozyme and designing an online recovery system for the production of human lysozyme by K. lactis K7 in biofilm reactor with plastic composite support. As a result, 25% silicic acid at pH 4 at 25ºC as the best adsorption conditions and 1 M NaCl+20% ethanol+5% sodiumdodecylsulfate at pH 6 at 25ºC as the best desorption conditions were selected for online human lysozyme recovery system (p<0.05). The online recovery phase is still in progress.
