P38 Solulys Advantages Compared to Commonly Utilized Industrial Yeast Extracts
Monday, July 21, 2014
Rongzhu Cheng1, Eric Lee1, Sebastien Maubourguet2, Antoine Billa2, Doug Wenzel1, Leon Zhou1 and Silvia Schnicker1, (1)Industrial Application, Roquette America Inc., Geneva, IL, (2)Roquette Frères, Lestrem, France
Solulys is not a “by-product”, rather a corn steep type product developed specifically by Roquette.  The tightly controlled manufacturing process is aimed at producing a characteristic product profile that differentiates Solulys from typical CSL.  Solulys demonstrates lot to lot consistency in composition1 including DS, protein, free amino acids, vitamin and minerals; predictable performance2, and industrial pricing/availability. 

In this study, we will demonstrate the performance of Solulys in both cell growth and enzyme productivity, compared to commonly utilized industrial yeast extracts.  A performance comparison of this rich source of nitrogen, amino acids and vitamins was completed by utilizing an alpha-amylase fermentation model with Bacillus subtilis (ATCC #21770), a yeast propagation study with S. Cerevisiae, and assorted other microorganisms. 

Results: (1) Solulys demonstrated improved buffering capacity, and favorably with yeast extract performance; (2) Specific yeast extracts demonstrated limitations in supplying vitamin/mineral content required in cell propagation; (3) Examination of cell growth with a range of common industrial microorganisms demonstrated improved cell growth when using Solulys vs. yeast extract; (4) Cell viability was dramatically improved in media containing Solulys.