Optimization of glucose release from taro peel waste for lactic acid bacteria cultivation

In the recent years, sustainable utilization of agricultural wastes have gained much attention due to the steady increased in the amount of solid waste generated from both field waste and manufacturing processes.  In this study, taro waste was utilized to produce glucose as carbon source for lactic acid bacteria (LAB) cultivation. Optimum parameters for maximum reducing sugar release from liquefaction were determined by Box-Behnken Design Response Surface Methodology (BBD-RSM). The optimum combination of temperature, α-amylase dosage, and time of reaction for liquefaction was determined as 79.2^oC, 0.9 mL of α-amylase, and 5 hr by using BBD-RSM, producing 30.57 g/L of reducing sugar. For optimum saccharification, 30 μL of amyloglucosidase, 60^oC, and 3 hr incubation resulted in approximately 60.14 g/L of glucose. The glucose produced from taro waste was blended with different agricultural wastes to produce a more economic and environmental friendly medium for LAB cultivation. The glucose concentration and the nitrogen source for maximum biomass production of Lactobacillus acidophilus BCRC 14079 was determined as 37 g/L with chicken meal (CM) and corn gluten meal (CGM), respectively. The Log CFU/mL of L.acidophilus BCRC 14079 produced by MRS medium, CM medium, and CGM medium were determined as 11.52, 10.34, and 10.83, respectively.