P91: Parallel Cultivation of E. coli in specifically adapted Single-Use Bioreactors

Single-use bioreactor solutions have been successfully established in animal and human cell culture in the last years. Now this technology is going to make its way into microbial applications. In the following case study demonstrates that reproducible process control could be achieved using single-use mini bioreactors operated in parallel. Fermentation of E. coli K12 resulted in reliable results proving the tested rigid wall stirred-tank single-use vessels to be appropriate for this particular microbial process development.

 

The study indicates that the BioBLU 0.3f single-use bioreactor addresses the specific needs of an E. coli fermentation including mass and heat transfer. The single-use design, featuring Rushton impellers, active heating and cooling, and overhead drive enabling high performance agitation, supports the particular, e.g. OTR and heat removal, demands of microbial applications. It is to be expected that single-use technology will lead to accelerated process development in fermentation.