S153: Refactoring natural product biosynthesis in bacteria

Naturally occurring fine chemicals exhibit unique properties that are valuable to many industrial applications. However, it remains challenging to produce these complex molecules via chemical methods or industrial fermentation. Pivot is developing technology to access and diversify gene clusters encoding fine chemical biosynthesis. We refactor the underlying genetics using synthetic regulatory circuits to enable algorithmic pathway engineering. We have established a pipeline for rapidly printing, debugging, and optimizing bacteria that express refactored metabolic pathways. In this presentation, we will discuss the application of our technology to non-ribosomal peptide biosynthesis.