P122: Free radical scavenging effect of various extracts of Annona atemoya Hort leaves by DPPH method

Monday, August 12, 2013
Pavilion (Sheraton San Diego)
Yeu-Ching Shi1, Ting-Chu Yu2, Kuan-Chen Cheng2, Ming-Hui Chen1 and Ching-Hui Chang3, (1)Eastern Specialty Industry Technology Department, ITRI South Campus, Taitung County, Taiwan, (2)Graduate Institute of Food Science Technology, National Taiwan University, Taipei, Taiwan, (3)Eastern Specialty Industry Technology Department, ITRI South Campus, Tainan, Taiwan
The Annona atemoya Hort is a hybrid of two fruits – A. squamosa and A. cherimola, which is the popular fruit in Taiwan, called pineapple sugar apple. Pineapple sugar apple leaves made a huge 5.2 tonnes of agricultural waste per year in Taiwan; it is interesting that if there are any functional ingredients in leaves. This study aimed to investigate the in vitro antioxidant properties of various extracts (alcoholic and aqueous) of pineapple sugar apple (Annona atemoya Hort) leaves. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was used for evaluation of antioxidant activity of extracts and butylated hydroxyanisole (BHA) was taken as standard. The leaf extracts (alcoholic and aqueous) showed antioxidant activity towards DPPH with EC50 values ranging from 60.0 to 1070 μg/mL. The antioxidant capacity determined by DPPH assay showed that 95% ethanol extract had the highest free radical scavenging activity with EC50 values of 60.0 μg/mL. The results demonstrated that alcoholic extract of Annona atemoya Hort leaves is a viable source of natural antioxidants and might be potent for functional foods. 

Key words: Annona atemoya Hort, pineapple sugar apple, DPPH radical scavenging activity