P44: Characterization of xylanase from a color variant Aureobasidium pullulans isolated in Thailand

Monday, August 12, 2013
Pavilion (Sheraton San Diego)
Wichanee Bankeeree1, Sehanat Prasongsuk1, Pongtharin Lotrakul1, Douglas E. Eveleigh2 and Hunsa Punnapayak1, (1)Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand, (2)Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, NJ
A number of xylanase-producing A. pullulans, both typical pigmented and color variant isolates, were obtained from different leaves of plant collected throughout Thailand. A color variant strain, 109B, was found to exhibit cellulase-free and thermo-activated xylanase activity. The highest xylanase yield (4.10 U.ml-1) was obtained in the medium containing 1% (w/v) corncob after 3 days of cultivation. The enzyme was purified, using ultrafiltration, ammonium sulphate precipitation (60-80% (w/v)), ion exchange and gel filtration chromatography, and was then characterized. Molecular mass of the enzyme was approximately 72 kDa as determined by SDS-PAGE and the optimal pH and temperature were 6.0 and 70oC, respectively. The metal ions, Fe2+ and Cu2+, partially inhibited the enzyme, while Mg2+, Co2+ and Ca2+ slightly enhanced the enzyme activity. Using beech wood xylan as the substrate, the Km and Vmax values of the enzyme were 11.71 mg.ml-1 and 2.05 µmol.min-1.mg-1protein, respectively. This enzyme was relatively stable at 50oC since it could retain more than half of its original activity after 3-hr incubation period. The thermostability of the enzyme was further improved using polyols (mannitol, sorbitol and glycerol). Addition of mannitol (0.5 mM) to the enzyme solution was revealed to be the best to prolong the half-life of this xylanase around 3.5-fold at 70ºC.