P34: Stabilized Enzyme microreactors and CO2 Bioconversion

Enzymes immobilized on nano/micro sized organic/inorganic hybrid materials have been known to be stabilized. For meeting the requirement of enzymatic processes, such as highly stabilized activity, enhanced specific activity with high enzyme loading, and easy scale-up, enzymes have been subjected to be aggregated on the surfaces of quantum dots-embedded nanofibers, organic-inorganic hybrid microspheres, or entraped in calcium phosphate nanoparticles via biomineralization as porous support materials for good accessibility of substrate. In this talk, successful immobilization of enzymes on these nano/micro sized organic/inorganic hybrid materials will be shown with some evidences obtained from various analysis methods. As results, the immobilized enzymes have shown the good storage and recyclable stabilities in terms of its enzyme activity and repeated usability, which resulteed in becoming stablized enzyme microreactors. In addition, a conceptual approach using enzyme microreactors is also shown to be possible for the synthetic pathway engineering.  Furthermore, considering enzymatic bioconversion of CO₂ into useful materials, it will be also presented how this enzyme stabilization technology developed here could be a key tool for realizing enzyme-based CO₂ bioconversion. Some examples of enzyme-based CO₂ bioconversion will be also presented with perspectives on its enzyme micro-reactor based CO₂ bioconversion.

REFERENCES

1.  J. of Materials Chemistry,21 (18), 6491 – 6493, (2011).

2. J. of Materials Chemistry, 21 (14), 5215-5218, (cover page article).(2011)

3. Green Chemistry, 14, 8, 2216-2220 (cover page article).(2012)

4. Engineering in Life Science, In Press, (2013).