P16: Light-triggered biocatalysis using thermophilic enzyme-gold nanoparticle complexes

This work is the first known attempt to synthesize well-defined thermophilic enzyme-gold nanoparticle complexes and to use them nonconventionally to run a conventional biochemical reaction. The complexes consist of A. pernix glucokinase (a central enzyme in glucose metabolism) conjugated through gold-thiol chemistry to gold nanorods, which are then encapsulated in ~2mm calcium alginate beads for thermal gradient control and reusability. Our data support the proof-of-concept that reaction rates can be enhanced without raising the bulk fluid temperature by using light to plasmonically heat the nanorods. Differing from other studies on remote-controlled reactions, this work describes a general materials strategy to control biochemical reactions with light. The results can conceptually be implemented into industrial biological processes, can lead to new applications such laser-assisted PCR and bio-hydrogen production, and can lead to a new way to study and regulate biochemical pathways in vivo.