P50: The genotype diversity of human norovirus detected in groundwater in korea

The Genotype diversity of human norovirus detected in groundwater in Korea In Sun Joo1, Jeong Su Lee1 , Yoo Jin Kim1, Yoo Eun Han1, Seok Jun Hwang1, Soon Ho Lee1, and Kun Sang Park1 Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation1 To investigate genetic diversity of human norovirus(NoV) detected from under groundwater in Korea, this study was performed from January 2009 to October 2012. We attempted to amplify NoV genome targeting ORF 1-2 junction region of viral genome by reverse transcription-polymerase chain reaction(RT-PCR) method. Subsequently automatic sequencing and sequence analysis using DNAstar software were performed for genotyping of NoV detected from groundwater. Then, we conducted a sequence alignment using reference strains of NoV and analyzed the nucleotide sequences of detected NoV strains to obtain the molecular epidemiological information on 134 sites where NoV was detected out of 8,454 sites in total. As a result of genetic prevalence of NoV in groundwater, GI-1(22%) accounted for the highest number of detected cases in the GI group, while GII-4(59%) had the highest number of detected cases in the GII group. The findings may be useful in providing the molecular epidemiological information on NoV in groundwater in South Korea.