Development of genetic systems for engineering and expression of secondary metabolite biosynthetic genes in actinomycetes: issues, challenges, and solutions
Richard H. Baltz, CognoGen Biotechnology Consulting, Indianapolis, IN

Genetic techniques and advances in the Archaea: implications for biofuel and natural product production strategies
Thomas J. Santangelo, Department of Microbiology and the Center for RNA Biology, The Ohio State University, Columbus, OH

Break

Genetic tool development and metabolic engineering of C. thermocellum for biofuel production
Ranjita Biswas¹, Daniel Olson², Lee R. Lynd² and Adam M. Guss¹, (1)Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, (2)Thayer School of Engineering, Dartmouth College, Hanover, NH

Methylation by cognate methyltransfersases is required for DNA transformation of Caldicellulosiruptor species: construction of mutants for the conversion of biomass to biofuels
Janet Westpheling, Genetics, University of Georgia, Athens, GA, and BioEnergy Science Center, Biosciences Division of DOE, Oak Ridge National Laboratory, Oak Ridge, TN

Tools for metabolic engineering of Scheffersomyces stipitis for improved fermentation of xylose and cellobiose
Thomas W. Jeffries¹, Sarah D. Mahan¹, Shawn S. Nelson¹, Jennifer R. Headman² and Laura B. Willis³, (1)Forest Products Laboratory, USDA Forest Service, Madison, WI, (2)Verdezyne, Inc., Carlsbad, CA, (3)Institute for Microbial and Biochemical Technology, USDA Forest Products Laboratory, Madison, WI