We have developed a new corn peptone, Amberferm 4500, which provides a reproducible source of nutrients for industrial fermentation. This peptone can be used in conjunction with the existing formulation to boost the growth of an organism or as a nitrogen source in fermentation. Comparative analysis shows that, AMF4500 provides about 20% more protein along with lower ash and moisture and overall more value added option. In comparison to corn steep powder, peptide molecular weight analysis shows that the products consists of small peptides, <5KDa in size. Free amino acid analysis shows that AMF4500 has four times more free amino acids on dry solids basis. We will report on the ability of AMF4500 to support growth of microorganisms in different growth media and also on its effects on the lag phase of growing organisms. A strong manufacturing specification has allowed us to develop a new reliable corn peptone as fermentation nutrient.