Monday, August 13, 2012
Columbia Hall, Terrace Level (Washington Hilton)
There are many techniques and tools used in strain selection and process development to speed up the research and development process. It is not only important to have the tools to conduct development but to also understand how those tools impact the development process. One tool that is often used in R and D is small scale high-throughput bioreactors because they allow for the cell lines to be screened controlled in both the microbial and mammalian world rapidly and earlier in the process with fewer resources. Typically small scale technologies often use different sensor technologies and mixing strategies compared to traditional larger scale vessels. A variety of engineering aspects of a small scale bioreactor will be explored which will include pH, temperature and dissolved oxygen control under multiple operating conditions (volume, mixing, and vessel configuration). We will also explore oxygen transfer rates not only in the growth phase but also in production phases when the culture are at high optical density. Data will also be shown where high-throughput strain selection and fermentation was conducted to deliver a robust process that was scalable using Pfenex’s Pseudomonas fluorescens expression system.