P74: RoboLector family – from automated media preparation to fed-batch fermentation in shaken microbioreactors

Bioprocess development currently begins with screening in simple and uncontrolled cultivation systems such as shake flasks or microplates, allowing researchers to gain only limited information. This situation changes with the application of microbioreactors. Microbioreactor platforms such as the BioLector use small arrays of micro vessels for culturing cells. The BioLector is based on microplates with the potential for high oxygen transfer rates (0.15 mol/L/h), allowing for improved consistency at large scale. The BioLector measures highly relevant fermentation parameters such as biomass, fluorescence, pH and DO values online in up to 48 parallel wells. Recently, this system was integrated into standard liquid-handling systems for automated media preparation and high-throughput experimentation. The RoboLector systems provide high-information content and automation to study bioprocesses more precisely and to dramatically reduce efforts and costs.

We will present data on the application of Design of Experiments (DoE) in media optimization and fed-batch fermentation. The whole factorial design was imported into the RoboLector software and applied for automated experimental set-up and execution. RoboLector results will be shown on media screening with microbial systems and fed-batch fermentations with Pichia pastoris comparing continuous glucose feedings with automated pulsed glycerol feedings, both induced with methanol. The results give detailed understanding into the bioprocess from microbioreactors that are difficult to achieve in bench scale laboratory fermenters.