P15: Effect of selected sugars and immobilization methods on the activity and stability of enzymes related to fruit ripening

Nitrile hydratase (NHase), amidase (AMD), 1-aminocyclopropane-1-carboxylate deaminase (ACCD), cyanidase, and β-cyanoalanine synthase-like enzyme (βCAS-like) activity in Rhodococcus rhodochrous DAP 96253 can be over expressed when induced with selected inducers such as cobalt and urea. These enzymes are correlated with delayed fruit. This work shows that supplementation of selected sugars such as trehalose and sucrose in growth media and storage buffers of R. rhodochrous DAP 96253 impacted enzyme activity and stability. The thermostability and osmostability of enzymes were examined under the protection of selected sugars. The effects of different immobilized catalyst on delaying the ripening of fruit were investigated. Both live (whole cell) catalyst and immobilized catalyst derived from induced cells have been shown to significantly delay the ripening of fruit.