Sunday, August 12, 2012
Columbia Hall, Terrace Level (Washington Hilton)
Sulfate-reducing bacteria (SRB) represent a class of anaerobic microorganisms that conduct dissimulatorysulfate reduction to obtain energy. The present study aimed to detect and control SRB activities using a very rapiddetectable culture medium and reduction of potential economic loss in the petroleum sector. This study is an attemptto isolate SRB from sea water by rapid and sensitive culture media and to control their effect using eight commercialbiocides (Aldehydes and quaternaries). The present work studies the effect of composition of four recommendedculture media (Postgate medium B, Starkey’s, Baar's and API media), besides, the presence of metal coupons inthese media to enhance the growth of sessile SRB. Furthermore, the present study evaluates the efficiency offiltration of these culture media on the growth of SRB. The results revealed that modified Postgate medium B wasthe recommended medium for SRB growth. In addition, the results showed that rapid and abundant growth of SRBwhen the metal coupons were immersed in the culture media which were deficient in iron. The unfiltered culturemedia improved the SRB growth. The growth of SRB was depressed by 15 ppm of the commercial quaternariesrather than 20 ppm of the aldehydes.