P78: Corn stover-enhanced cellulase production by Aspergillus niger NRRL 567

Monday, July 25, 2011
Grand Ballroom, 5th fl (Sheraton New Orleans)
Sibtain Ahmed1, Mohammad Ishfaq Gori2, Mohammad Aslam Malana2 and Amer Jamil3, (1)University of New Mexico, Albuquerque, NM, (2)Department of Chemistry, Bhauddin Zakaria University, Multan, Pakistan, (3)Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan
The production of extracellular cellulases by Aspergilus niger NRRL 567 on corn stover was studied in liquid state fermentation. In this study, three cellulases, exoglucanase (EXG), endoglucanase (EG) and b-glucosidase (BGL) were produced by A. niger NRRL 567. The optimal pH, temperature and incubation time for cellulases production was found to be 3.5, 30 oC and 96 h respectively. Maximal cellulases activities were achieved with 4 % corn stover, 0.1% molasses and 1% yeast sludge. The present research indicated that A. niger effectively produced celluases and could be utilized for industrial production of cellulases. The possibility of using locally available substrates such as corn stover and yeast sludge for cellulases production was promising. To our knowledge this is the first report on production of cellulases by using corn stover as a substrate from A. niger NRRL 567.
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