Monday, July 25, 2011
Grand Ballroom, 5th fl (Sheraton New Orleans)
C. acetobutylicum BKM19 is a hyper-alcohol-producing derivative of C. acetobutylicum PJC4BK, a buk knockout strain. In this study, the plasmid pIPA3-Cm2, which harbors a synthetic acetone operon (adc-ctfA-ctfB) and a primary/secondary alcohol dehydrogenase from C. beijerinckii NRRL B-593 was introduced to C. acetobutylicum BKM19 to establish IBE-producing strain. This strain was used in fed-batch fermentation experiments with in situ removal of solvents. Consistent with a previous study using C. beijerinckii, fed-batch fermentation could not be prolonged without appropriate supplementation of nutrients other than glucose. By feeding ammonia and other nutrients, the fed-batch fermentation had been operated for more than 200 hours and ca. 600 g/L of glucose was completely consumed. [This work was supported by the Advanced Biomass R&D Center of Korea (ABC-2010-0029799) through the Global Frontier Research Program of the Ministry of Education, Science and Technology (MEST). Further support by GS Caltex, BioFuelChem, EEWS program of KAIST, and the World Class University program (R32-2008-000-10142-0) of the MEST are appreciated.]