S106: Design of a ketoreductase platform for biocatalysis in the pharmaceutical industry

The use of ketoreductase enzymes to produce chiral alcohol intermediates is a valuable tool for process chemists in the pharmaceutical industry. While there are commercial sources of these biocatalysts available; for reasons of expanded substrate scope and access to low cost biocatalysts, we here describe a platform of ketoreductase enzymes that can be
produced at low cost and with freedom to operate.  This collection of enzymes consists of biocatalysts from three sources: (1) Wild type and mutant enzymes identified through database mining and literature searches, (2) Mutants of enzymes made during enzyme engineering efforts, and (3) Enzymes discovered while sequencing the genome of a bacterium.