S64: Antigenic characters in mixed culture of some bacteria isolated from Nile water and  Kasr Al Ayiny hospital  effluent in Cairo

Monday, July 25, 2011: 3:30 PM
Nottoway, 4th fl (Sheraton New Orleans)
Amber S. Gad Jr., Chemistry of Natural and microbial products, National Research Center, Cairo, Egypt, Gamal A. Osman, Water Pollution Dept, National Research Center, Cairo, Egypt and Helmy M. Hasan, Microbial Chemistry Dept., National Research Center, Cairo, Egypt
The main aim is to prove that  chlorination resistance and antibiotics resistance and sensitivity triats were transfered from kasr El Ayiny hospital effluent to Nle water isolates when co-cultured for 48 h at 30°C under normal laboratory condoitionsc and to find some new pre- teatmnt before the disposal in aquatic environment as it has a hazardous effect on public health. Also,.the tap water total count werefound to be more reistant to antibiotcs than the isolates in the water treatment plant ,this is because of the inferiority in  the distribution system and the evolution of ± gram bacteria.Also tap water is free from antibiotics whereas the hosptl effluent isn't.Results showed that staphlococcus aureus,Pseudomonas aueroginosa,and Streptococcus faecalis converted to reistant isoltes when incubated with streptococcus fecalis,,E.coliand Staphylococcus aureus respectively.The tested antibiotics were naldixic acid,30mg/l;gentamyicin,10mg/l;ampicillin,30mg/l and amoxycillin30mg/l.These bacteria were sensitive to erythromycin,15mg/l,tetracyclin.30mg/land ;rifpimicin,50mg/l The isolates were also resistant to chlorination up to 120 min in 5ppm dose.However Salmonella typhi and E. coli isolates from Nile water were senstive to both antibiotics and 5ppm chlorine in pure and mixed culture.