S146: Genomes, Pathways and Metabolic Reconstructions for Microbial Engineering

Whole genome sequencing, annotation, pathway integration computational tools have not only accelerated product development but has helped create new strains for product production or protein expression.  Efficient production not only requires tracking of host gene (s) expression to accurately map chemical path to product but also to identify metabolic sinks that affect production levels.  Metabolic reconstructions are also valuable for optimal growth media design for biomass production and cost minimization. Integrated Genomics provides such a platform for integration of genomic data, annotations, pathways and expression data for over 1,800 genomes including biofuels, probiotic and fine chemical producing bacteria.  As a case study, we have determined whole genome expression using transcriptional arrays of E .coli under laboratory growth conditions.  Results of the metabolic compounds were mapped to their corresponding genes and pathway data in ERGO.   This approach could be used for growth under small scale fermentation, under production conditions as well as for metabolic engineering.