Monday, August 2, 2010
Pacific Concourse (Hyatt Regency San Francisco)
Trehalose (α-D-glucopyranosyl α-D-glucopyranoside) is a disaccharide contains no reducing sugar. Generally, trehalose is known as energy source and protective agent in organism against environmental stresses. It is found in nature, ranging from bacteria to plants and animals. In present, trehalose was synthesized by five different mechanisms using starch, maltose and glucose-6-phosphate as substrates. Maltoologosyltrehalose synthase, trehalohydrolase (MTSH) is a fusion enzyme which combines with maltooligosyltrehlose syntahse (MTS) and maltooligosyltrehalose trehalohydrolase (MTH). These enzymes produce maltooligosyltrehalose and trehalose, respectively. As a result, MTSH can produce trehalose by one step from oligosaccharide. Amylosucrases (AS) hydrolyze sucrose to glucose and fructose, then make α-1,4 glucan from released glucose. In this research, we produce trehalose by double enzyme reaction with MTSH from Brevibacterium helvolume, and AS from Deinococcus geothermalis by using sucrose as a sole substrate. Trehalose production increased depending on glucose concentration, reaction time and amount of MTSH.