Monday, August 2, 2010: 9:00 AM
Bayview A (Hyatt Regency San Francisco)
Frank Baganz, Department of Biochemical Engineering, The Advanced Centre for Biochemical Engineering, London, United Kingdom
The need for greater speed during the development of fermentation and cell cultivation processes has focussed much attention onto the development and application of miniaturised and high throughput devices [1]. In this presentation I will introduce technologies for parallel bioprocess development focussing on shaken microwell systems and miniature stirred tank reactors. I will discuss the engineering characterisation in terms of power input, liquid phase mixing and oxygen mass transfer [2] and give examples for the application of these technologies in fermentation and cell culture process development and scale-up [3, 4].
[1] Betts J.I. and Baganz F. (2006) Miniature Bioreactors: current practices and future opportunities. Microbial Cell Factories 5: 21.
[2] Micheletti M., Barrett T., Doig S.D., Baganz F., Levy M.S., Woodley J.M. and Lye G.J. (2006) Fluid mixing in shaken bioreactors: Implications for scale-up predictions from microlitre scale microbial and mammalian cell cultures. Chem. Eng. Sci. 61: 2939-2949.
[3] Gill N.K., Appleton M., Baganz F. and Lye G.J. (2008) Design and characterisation of a novel miniature bioreactor system for parallel microbial fermentations. Biochem. Eng. J. 39: 164-176.
[4] Silk N.J., Denby S., Lewis G., Kuiper M., Hatton D., Field R., Baganz F. and Lye G.J. (2010) Fed batch operation of an industrial cell culture process in shaken microwells. Biotechnol. Lett. 32: 73-78.
