S134: Expeditious strain and scalable fermentation development for quality recombinant protein production in Pseudomonas fluorescens

The ability to produce a high quality protein product in a time and cost efficient manner is of particular value throughout the discovery to commercialization pathway of therapeutic and vaccine proteins.  To this effect, a suite of toolboxes spanning strain, analytics, fermentation, and downstream process development have been established for a Pseudomonas fluorescens based expression platform.   An off-the-shelf toolbox of expression plasmids with a wide range of gene expression strategies and host strains with diverse phenotypes, in conjunction with high throughput, parallel screening methods, combine seamlessly to deliver robust protein expression strains.  Coupled with simultaneous strain and far-reaching fermentation evaluation in a mini-bioreactor system which can be predictably scaled, high levels of soluble, properly folded protein very often result.  Case studies of successful implementation of this platform and their subsequent scale-up will be presented.