P2: A NOVEL RAW STARCH HYDROLYSING AND MALTOTETRAOSE PRODUCING AMYLOLYTIC ENZYME FROM GLUCOMYCES SP. A1

An extracellular amylolytic enzyme from a Glucomyces sp. A1 producing maltotetraose on starch hydrolysis revealed the ability to hydrolyze raw native starches from various sources. The amylase, purified 3.2 olfd by a combination of Ion exchange on Q-Separose, hydrophobic interaction on phenyl Sepharose 6 fast flow (high sub) and gel filtration chromatographies using Sephadex G-75 with a 25% enzyme yield, had a molecular weight of 240KDa as determined by SDS-PAGE electrophoresis. The amylase activity was optimal at 40oC and pH 7. Chromatographic determination on hydrolysis of starch and various poysaccharides indicates the production of glucose, maltose and maltotetraose